Los centros Fe-S se pueden seguir por EPR, Mossbauer, o Raman

• A, EPR spectra of the reconstituted GST-fused N1c fragment (upper) and the isolated non-fused N1c fragment (lower) at 12 K in the presence of 10 mm sodium dithionite.

• Eiko Nakamaru-Ogiso et al. J. Biol. Chem. 2002;277:1680-1688

• ©2002 by American Society for Biochemistry and Molecular Biology

Resonance Raman spectra of purified -bound form of NifIscA. Recorded at 17 K with λex 457.9, 487.9 and 514.5 nm. Sample ~2 mM in [2Fe-2S]2+ clusters).

Mapolelo, D. T. et al. Biochemistry (2012) 51: 8071-84 , Doi: /10.1021/bi3006658

Notes:

Además, mediante espectroscopia de EPR y Raman es posible investigar los el estado Redox de los centros Fe azufre unidos a proteínas purificadas.